Isolation and identification of compounds from Phaleria macrocarpa (Scheff.) Boerl fruit extract

ABSTRACT

Objective:To identify and isolate the chemical compounds of Phaleria macrocarpa (P.macrocarpa) fruit ethanolic extract.Methods:Dried fruit of P.macrocarpa was extracted with 90％ ethanol and partitioned between n-hexane/H2O and ethyl acetate/H2O.The organic layer was fractionated by various stationary phase and identified by using combined data of ID [(proton nuclear magnetic resonance (NMR),carbon-13 NMR)],2D-NMR (heteronuclear multiplequantum correlation and heteronuclear multiple-bond correlation),and mass spectrum.Results:Purification of n-hexane and ethyl acetate fractions from ethanolic extract of P.macrocarpa fruit resulted in isolation of nine compounds.Conclusions:A new compound was isolated and identified as glyceryl pentacosanoate.Also,two xanthones,which are 1,7-dihydroxy-3,6-dimethoxyxanthone and 1,6,7-trihydroxy-3-methoxyxanthone,are firstly reported to be isolated from P.macrocarpa.