INVESTIGATION ON THE DISTRIBUTION OF ESTERASE D(EsD)PHENOTYPES AND THE DETECTION OF EsD FROM BLOODSTAINS / 中国法医学杂志

ABSTRACT

The phenotyping of erythrocyte esterase D of 221 Chinese random donors,Han nationalities in Shenyang,China,was performed by the agarose gel electrophoresis.Gene frequencies wereE_SD~1=0.629,E_SD~2= 0.371.There is no significant difference of gene frequencies of Han nationalities between Shenyang and Beijing.The minimal amount meeted the requirment for detection of E_S D were 1?l of fresh hemolysate or 1?l hemolysate.The E_S D can be detected from the hemolysates kept in 37℃ for 7～8days as well as from 1?l hemolysate bloodstain kept for 2 weeks and 5?l hemolysate bloodstain kept for 3 weeks.