Determination of methaqualone and 2-methyl-3[2-(hydroxymethyl) phenyl]-4 (3H)quinazolinone concentrations in human serum by RP-HPLC / 中国法医学杂志

ABSTRACT

Methaqualone and 2-methyl-3 [2- (hydroxymethyl) phenyl]- 4 (3H) quinazolinone (metabolite I) concentrations in human serum wereanalyzed quantitatively by reverse high performance liquid chromatography (RP-HPLC). The concentrations of methaqualone ranging from 1-35?g/ml were in good linear relationship(Correlation coefficient r = 0. 9980, regression line y = 0. 06324x-0. 1029), The average recovery of methaqualone reached as high as 102.08?9. 987(SD)% (n= 5). The detective limit was 1 ng. The relative quantity of metabolite I was determined according to the linear concentration of methaqulone. RP-HPLC assay is proved to be useful in determening blood methaqualone and metabolite I concentrations in forensic toxicology.