Effect of verapamil on the alteration of phospholipase A_2 activity in acute pancreatitis rats / 中国普通外科杂志

ABSTRACT

Objective To evaluate activity alteration of the pancreatic tissue phospholipase A 2 (PLA 2) and the therapeutic effects of verapamil in acute pancreatitis (AP) rats. Methods After the model of AP in the rat was established by the closed duodenal loop technique, the changes of PLA 2 activity were observed, and the pancreatic histology was examined by light and electron microscopy. Results At 16 and 24 hours after induction of AP in rats, significant inhibition of PLA 2 avtivity in pancreatic tissue was shown in the treated group (32.34?3.87, 35.26?4.52) as compared with AP group (44.83?5.31,47.77?5.86). The treated animals also showed decreasing in the severity of pancreatic pathology. Conclusions In AP rats exist high activity of PLA 2. Verapamil, a calcium channel blocker, might have therapeutic effects on AP by inhibiting the activity of PLA 2.