Role of NF-?B in inducing HEL cell proliferation and apoptosis during human cytomegalovirus infection / 中国病理生理杂志

ABSTRACT

AIM: To investigate whether human cytomegalovirus(HCMV) regulate human embryonic lung fibroblast(HEL) cell proliferation and apoptosis by activating NF-?B. METHODS: Immunohistochemistry and Western blot analysis were used to detect the NF-?B translocation and/or Bcl-2 and the levels of I-?B? during HCMV infection. Apoptotic cell were examined by flow cytometry, and the HEL cell proliferation was determined by MTT. RESULTS: The levels of NF-?B in the nucleus reached highly 48 h postinfection, and the levels of I-?B? were low 24 h postinfection. The activity of NF-?B was inhibited 120 h postinfection. The levels of bcl-2 was accorded with the activity of NF-?B. HCMV promoted HEL cells to proliferate before 72 h postinfection and induced apoptosis 120 h postinfection. CONCLUSION: NF-?B plays a role in HEL cell proliferation and apoptosis during HCMV infection, and it involves in the pathological mechanisms of diseases associated with HCMV infection.