Antioxidant and serum lipid lowering effects of luffa cylindrical on experimental hyperlipidemia mice / 中国病理生理杂志

ABSTRACT

AIM:To observe the antioxidation and the serum lipid lowing effects of polyphenols in luffa cylindrical on experimental hyperlipidemia mice.METHODS:Acetone was used to extract polyphenols from luffa cylindrical.The content of polyphenols was determined by Folin-ciocalteau method.The inhibitory efficacy of the extracts from luffa cylindrical to the production of OH? free radical was measured.Kunming mice were used to establish the hyperlipidemia model by feeding high fat diet.The freeze-dried fresh luffa cylindricals was added to the treated animals,xuezikang was also used as a positive control.After feeding high-fat food for 14 days,serum were collected to measure the total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C)and superoxidedismutase(SOD).The liver tissue was also collected for determining malonaldehyde(MDA)content.RESULTS:(1)Content of polyphenols in fresh luffa cylindrical was(0.3?0.1)g /kg.(2)The polyphenol extracts from fresh luffa cylindrical had a strong efficacy to inhibit the production of OH? free radicals.(3)The trend of body weight gain in groups LC1(low dosage luffa cylindrical)and LC2(high dosage luffa cylindrical)became slow down obviously,compared with that in group HPL(high-lipid model).(4)The levels of TC(4.19?0.37)mmol/L and LDL-C(2.77?0.79)mmol/L in group LC1,TC(3.56?0.55)mmol/L and LDL-C(2.34?0.41)mmol/L in group LC2,TC(4.59?0.96)mmol/L and LDL-C(3.25?0.67)mmol/L in group PC(positive control)were all lower than those in group HPL(P