Effect of blocking chloride channel on the proliferation and apoptosis of Hep-2 cell line / 中国耳鼻咽喉头颈外科

ABSTRACT

OBJECTIVE To study the effect of blocking chloride channel on the proliferation and apoptosis of Hep-2 cell line.METHODS Hep-2 cell line was used as a target,The effect of chloride channel blocker(NPPB)on the proliferation of Hep-2 cell line was evaluated by MTT assay.Flow cytometry was performed to measure the apoptosis index of selected Hep-2 cell clones.RESULTS Inhibition of the proliferation of Hep-2 cells depended on the dose of chloride channel blocker(NPPB).The apoptosis index of Hep-2 cells was remarkably different before and after its chloride channel was blocked. CONCLUSION Normal expression and function of chloride channel is essential for the maintenance of proper cell growth.Our results suggest that blocking the chloride channel could inhibit the proliferation and induce apoptosis of Hep-2 cell line.