Change of Gq protein-phosphatidyl inositol signaling in brain of rats with acute respiratory distress syndrome / 中国病理生理杂志

ABSTRACT

AIM: To study the change of Gq protein-phosphatidyl inositol signaling in the brain of rats with acute respiratory distress syndrome(ARDS).METHODS: Forty male Wistar rats were randomly divided into oleic acid groups(OA groups of 30 min,60 min,90 min and 120 min exposure) and control group.The ARDS model in rats was reproduced by intravenous injection of oleic acid(0.2 mL/kg in 2 min).The mean arterial blood pressure(MABP),blood gas indexes,malondialdehyde(MDA),lactate dehydrogenase(LDH) and creatine kinase(CK) in plasma and brain tissues were measured.G?q/11 protein and phospholipase C in brain tissues were detected by Western blotting.RESULTS: Compared to control group,MABP and PaO2 in all OA groups obviously decreased(P