Regulation of TNF-like cytotoxic facor(s) production by murine peritoneal macrophage / 中国免疫学杂志

ABSTRACT

Effects of the activiating and stimulating signals on the production of TNF-like cytotoxic factor (s) [Macrophage-derived cytotoxic factor(s), M?-CF] were investigated. Although activiating signals (Calcium ionophore A23187 and Macrophage-activiating factor, MAF) did not induce the production of M?-CF by themselves, they were significantly synergistic in the production of M?-CF induced by stimulators. Upon exposing to Iipopolysaccharide (LPS) M?-CF appeared in culture supernatant within 2 hrs, reached a peak after about 8 hrs and then declined gradually. In addition to LPS, opsonized zymasan A and several tumor cell lines also induced M?-CF production. whereas latex beads and normal allogenic spleen lymphocytes had no effect on the production. Finally the stimulating role of LPS, zymosan A and tumor ceils could not be mimicked by protein kinase C (PKc) activiators (phorbol myristate acetate, PMA and calcium ionophore A23187). These results suggested that the induction of M?-CF production be stimulus-specific and PKc-independent.