Studies on Characteristics of Heteromyeloma Cell Line HM—KMC1 / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)1985.
Article
in Chinese
| WPRIM
| ID: wpr-674653
ABSTRACT
Establishment of an ideal partner is one of the most important subject of human monoclonalantibody researches.At Present,the human-mouse hybrid myeloma cell line has more advan-tages over plasmacytoma and lymphocyte cell lines.In this subject,heteromyeloma cell line HM—KMC1 was derived from fusion by using murine myeloma SP_(2/0)—AG_(14) with healthy adult pe-ripheral blood lymphocytes that was induced to be a 8—Ag resistant and HAT sensitive cell lineafter a serious cultivation in RPMI1640 complete medium containing 8—Ag,then fused with Blymphocytes from human spleen,tonsil,lymph nodes and peripheral blood,respectively,Six fu-sions have been done successfully and the fusion frequency was 41—129/10~7 cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
1985
Type:
Article
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