Search for Gene Expression in Cervical Squamous Cell Carcinoma using GeneFishing(TM) DEG PCR Technique / 대한산부인과학회지
Korean Journal of Obstetrics and Gynecology
;
: 617-627, 2005.
Article
in Korean
| WPRIM
| ID: wpr-67468
ABSTRACT
OBJECTIVE:
The aim of this study was to investigate the gene expression profiles using GeneFishing(TM) DEG kit in Korean women with cervical squamous cell carcinoma.METHODS:
Cervical cancer biopsies were obtained from patients at the Department of Obstetrics and Gynecology, St. Mary's hodpital. In this study, we used a common reference that was mixed with an equal amount of RNA extracted from non-cervical cancer patients. The profiles of expression genes between cervical normal and squamous cell carcinoma tissue were identified using GeneFishing(TM) DEG Kit and screened by BLAST search.RESULTS:
Almost 100 differential expressed genes were identified in universal control and cervical squamous cell carcinoma, 53 of differential expressed genes, up-regulated expression of 32 and 21 down-regulated expression was sequenced. Up-regulated genes were calcylin, calgranulin A, TRK oncogene, HLC5, fibrillarin, collagene type I alpha1 etc. and down-regulated genes were galectin 1, PRP8 pre-mRNA precessing factor 8 homology, clusterin etc.CONCLUSION:
We identified gene expression profile in cervical squamous cell carcinoma using GeneFishing(TM) Kit in Korean women. The functional genomics of these genes should be further studied.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oncogenes
/
Biopsy
/
RNA
/
RNA Precursors
/
Carcinoma, Squamous Cell
/
Gene Expression
/
Uterine Cervical Neoplasms
/
Polymerase Chain Reaction
/
Collagen
/
Genomics
Type of study:
Prognostic study
Limits:
Female
/
Humans
Language:
Korean
Journal:
Korean Journal of Obstetrics and Gynecology
Year:
2005
Type:
Article
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