Study of IFN? effect on HL-60 cells or ATRA resistant HL-60 cells / 中国免疫学杂志

ABSTRACT

Objective:To detect in vitro the effect of IFN? on HL 60 cells or ATRA resistant HL 60 cells Methods:MTT was used to measure the proliferation of HL 60 cells or ATRA resistant HL 60 cells The differentiation of HL 60 cells or ATRA resistant HL 60 cells was detected by NBT To detect the apoptosis of HL 60 cells or ATRA resistant HL 60 cells with Flow Cytometric Analysis Otherwise,the ATRA resistant HL 60 cells was induced by way of ATRA density gradually increasement Results:IFN? could inhibit the proliferation of HL 60 cells,especially accompanied with the ATRA IFN? not only induce the differentiation but also promote the differentiation of HL 60 cells or ATRA resistant HL 60 cells ATRA resistant HL 60 cells post treatment with IFN? were sensitive to ATRA Conclusion:Effect on differentiation of HL 60 cells was enhanced by IFN? IFN? also reverse the resistance of ATRA resistant HL 60 cells to ATRA