Loss of heterozygosity on chromosome 6 in glioblastoma / 中国癌症杂志

ABSTRACT

Purpose:20 loci on chromosome 6 were examined to detect loss of heterozygosity(LOH)in 21 cases of glioblastoma(GBM) in order to locate the deletion areas probably harboring tumor suppressor genes.Methods:PCR based microsatellite polymorphism analyses were performed to detect LOH on chromosome 6, fluorescence labeled primers and Perkin Elmer 377 DNA Sequencer were applied.Results:47.6% informative cases of GBM displayed LOH on chromosome 6, 28.1% of informative loci showed LOH in our series, in which the most frequent LOH were observed at locus D6S281 (50%) on 6q tel and D6S287(50%) on 6q 16.3 ,the LOH frequency at locus D6S276 on 6p 21.1 21.3 is also high(35.3%).Conclusions:Loss of genetic material on chromosome 6 may be involved in the molecular genetic pathogenesis of GBM. The chromosomal regions at loci D6S281 on 6q tel ,D6S287 on 6q 16.3 and D6S276 on 6p 21.1 21.3 may harbor tumor suppressor genes associated with GBM. [