Micronucleus Test of Mice Peripheral Blood Reticulocytes Induced by Organic Extracts of Air Particulate Pollutants in Railway Transport Station of Chemical Dangerous Goods / 环境与健康杂志

ABSTRACT

Objective To investigate the micronucleus frequency in mice peripheral blood reticulocytes induced by air particulate pollutants in a railway transport station of chemical dangerous goods (RTSCDG) Methods Male BALB/C mice were selected and randomly divided into exposure groups and control groups One time exposure experiment was carried out among mice exposed to acetone extracts of air particulate pollutants at doses of 20,40,80,160 mg/kg by intraperitoneal injection Multiple time exposure experiment was carried out among mice exposed to acetone extacts of air particulate pollutants at dose of 10 mg/kg by consecutive intraperitoneal injection one time per day for 5 days The peripheral blood was sampled from mice tails and the micronucleus frequencies in reticulocytes were observed at 24, 48 and 72 h after the exposure Results The frequencies of micronuclei in peripheral blood reticulocytes revealed good time response relationship and reached the highest levels at 48 h after exposure,at the same time the frequencies of micronuclei in dose groups of 20,40,80,160 mg/kg were all higher than those in control group The multiple frequencies exposure experiment with lower dose showed that the frequencies of micronuclei in peripheral blood reticulocytes in exposure group always revealed significantly higher levels compared with those in control group during the 24th~72th hour after the exposure ( P