Study on Enzymology and Morphology Effects of Extractions of Cyanobacteria on Primary Cultured Hepatocyte / 环境与健康杂志

ABSTRACT

Objective To make a further study on the character and mechanism of hepatotoxic effect of microcystin(MC),which stands for the extractions of cyanobacteria Methods The enzymology and morphology effects of different concentrations of 0.1,1,10 ?g/ml extractions of cyanobacteria on primary cultured hepatocyte were observed Results It was shown that the concentrations of LDH and AST increased in the culture medium after exposure to MC at concentrations of 0.1 ,1 and 10 ?g/ml No significant changes of concentrations of AKP, GGT, ALT and GSH were observed in the culture media of tested groups.An active proliferation of cultured hepatocytes with apopotosis or necrosis were observed in the center of the proliferation foci in the treated groups.Characterisitic morphological alterations such as irregular shape,cytoplasmic cavity,plasma membrane blebs and protrusions were viewed in scanning electronic microscope as well as in cultured cell. Conclusion The extractions of cyanobacteria could promote the proliferation of hepatocyte and might influence the physiological and biochemical function and the integrity of hepatocyte