Preliminary study on the relationship between the immunostimulatory effects and the number of ISS in CpG DNA / 中国免疫学杂志

ABSTRACT

Objective:To investigate the relationship between the immunostimulatory effects and the number of immunostimulatory sequence(ISS) in CpG immunostimulatory DNA.Methods:Mice were immunized by HAV inactive vaccine together with three kinds of CpG ODN which contains 2,4,8 ISS respectively by intraperitoneal injection. At the weeks 4,6,8 after immunization, peripheral blood was obtained from the tail vein of mice and assayed for anti HAV IgG by the fully automated microparticle enzymeimmunoassay.Results:The immunogenicity of HAV inactive vaccine can be boost up by CpG ODN which contains 2 ISS, CpG ODN which contains 4 ISS did not show any immunostimulatory effect, act as an adjuvant, but CpG ODN which contains 8 ISS showed inhibiting the immunological effect of the vaccine.Conclusion:Adequate length of CpG ODN(when it contains 2 ISS) can act as an effective immunostimulator adjuvant, and it might decrease the immunogenicity of the vaccine if it contains much more ISS.