Change of mitochondria membrane potential of HL-60 cells induced by horseshoe crab hemocyte peptides / 中国癌症杂志

ABSTRACT

Purpose:Study the changes of mitochondria membrane potential of HL 60 cell induced by horseshoe crab hemocyte peptides. Methods:HL 60 cells were treated with horseshoe crab hemocyte peptides 0,12.5,25,50,100 ?g/ml for 2,4,6,8,10,12,14h. HL 60 cells were stained with Rhodamine123 before mitochondria membrane potential was detected. Change of mitochondria membrane potential of HL 60 cell was analyzed by flow cytometry. Results:Mitochondria membrane potential of HL 60 cells was stable from 0 to 14h in control groups,however mitochondria membrane potential was decreased when HL 60 cells were treated with horseshoe crab hemocyte peptides 12.5～100 ?g/ml for 0 to 14h.Conclusions:Decrease of mitochondria membrane potential was dependant on concentrations of peptides and time of peptides treatment, it was relative between horseshoe crab hemocyte peptides inducing HL 60 cells apoptosis and decrease of mitochondria membrane potential.