Ameliorate chemokine RANTES expression by the recipe of LangChangFang in murine lupus nephritis model / 中国免疫学杂志

ABSTRACT

Objective:To investigate chemokine RANTES(Regulated upon Activation.Normal T cell Expressed and Secreted)expression in the chronic graft versus host disease(GVHD) murine lupus nephritis model and effect of the recipe of LangChuangFang on it.Methods:A microwave based immunohistochemistry(SP) and RT PCR were used to detect the expression of RANTES in the kidney of model,therapeutic group and normal control.At the same time,the correlation between expression of RANTES and renal injures in model was examined.Results:LangChuangFang protected renal function,which was coincided with amelioration of pathologic lesions especially tubulointersitial injury.This was associated with the amelioration of RANTES protein and mRNA expression in model.Conclusion:RANTES involved in the process of renal injury in vivo,LangChuangFang can treat lupus as immunosuppressive agent by decreasing RANTES production.