Preparation and determination of polyclonal antibody to pre-mRNA splicing protein Tra2? / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)2001.
Article
in Chinese
| WPRIM
| ID: wpr-675150
ABSTRACT
Objective:
To prepare specific antibody against the pre mRNA splicing protein Tra2? in human fetal tissues.Methods:
A specific antigen fragment of human Tra2? was cloned into the pGEX 3x expression vector to express a recombinant GST Tra2? fusion protein.The fusion protein was expressed,purified and used as an antigen to immunize the New Zealand rabbit,and the polyclonal antibody to Tra2? was obtained.Results:
The antibody was successfully used to detect Tra2? protein in human fetal cerebrum with Western blot and immunohistochemistry analysis.Conclusion:
This prepard antibody has good specificity and can be used to detect the Tra2? protein in the human fetal tissues.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2001
Type:
Article
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