The effect of Ureaplasma Urealyticum infection on the IL-6 and TGF-?_1 secretion by rat Sertoli cells / 中国免疫学杂志

ABSTRACT

Objective:To investigate the role of immune regulation of the rat Sertoli cells in the infection Methods:The testis of the SD rat was digested by Collagenase (typeⅡ) and hyaluronidase, after filtrating and centrifugalizing, the rat Sertoli cells (higher purity) were obtained After Sertoli cells (in vitro) were infected by living Ureaplasma Urealyticum (UU)?supernatant without UU and heat inactivated UU, the effect of UU infection on the IL 6 and TGF ? 1 secretion by rat Sertoli cells were analysed by ELISA methods Results:The living UU and the supernatant without UU, in low dose, increased the IL 6 secretion by rat Sertoli cells (P