Effect of Ly49A transfected lymphocytes on graft versus host disease and graft versus leukemia effect after allogeneic bone marrow transplantation / 中华器官移植杂志

ABSTRACT

Objective To observe the effect of Ly49A transfected lymphocytes on graft versus host disease (GVHD) and graft versus leukemia (GVL) effect post allogeneic bone marrow transplantation. Methods Ly49A gene was transfected into lymphocytes of C57BL/6 mice by retrovirus and the expression rate of Ly49A receptor was detected by flow cytometer. The murine model of allogeneic acute GVHD was established in this way the donor was C57BL/6(H 2 b). The recipient was BALB/c(H 2 d), which was injected EL9611 cells. After the irradiation (TBI, 60 Co 9.0 Gy) the recipient accepted the transplantation of mixed spleen cells and bone marrow cells to establish the GVHD model. The effects of Ly49A transfected spleen cells on GVHD and GVL post allogeneic bone marrow transplantation were detected. Results The expression rate of Ly49A receptor was ( 42.20 ? 4.87 )?% for lymphocytes transfected with pLXSN Ly49A; ( 18.67 ? 2.48 )?% for lymphocytes transfected with pLXSN and ( 18.73 ? 3.82 )?% for untransfected control respectively. In the case of allo BMT (C57BL/6 H 2 b→BALB/c H 2 d), survival time was ( 6.50 ? 2.41 ) days for the irradiation group; ( 20.90 ? 2.88 ) days for cyclophosphomide therapy group; ( 17.10 ? 4.65 ) days for bone marrow cells mixed with lymphocytes transplantation group; ( 17.40 ? 5.32 ) days for bone marrow cells mixed with pLXSN transfected lymphocytes transplantation group; ( 35.20 ? 12.52 ) days for bone marrow cells mixed with Ly49A transfected lymphocytes transplantation group, which was much longer than the survival time of any of the above group ( P = 0.000 ). Conclusion The Ly49A transfected lymphocytes transplantation could alleviate GVHD and retain GVL effect on acute GVHD model post allo BMT.