The effect of allogeneic MHC Ⅰ gene modification on the immunologic character in bone marrow cells / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)1985.
Article
in Chinese
| WPRIM
| ID: wpr-675426
ABSTRACT
Objective:
To observe the effect of allogeneic MHC Ⅰ gene modification on the immunologic character in bone marrow cells and to investigate the mechanism of induce immunologic tolerance of MHC Ⅰ.Methods:
The retroviral expression vector of BALB/C mice H 2D d gene was constructed and transducted into C57BL/6 mice bone marrow cells And the expression of H 2D d on the infected cells was detected by FACS technique Then observe the MLR between the BALB/C mice spleen T cells and the C57BL/6 mice bone marrow cells modified with H 2D d gene by MTT assay The cytotoxic activity of BALB/C mice NK cells to the C57BL/6 mice bone marrow cells modified with H 2D d gene was detected by LDH release assayResults:
Either stimulation or response ability of the C57BL/6 mice bone marrow cells to the T cell from BALB/C mice spleen was significantly decreased after modified with H 2D d gene The cytotoxic activity of BALB/C mice NK cells to the C57BL/6 mice bone marrow cells modified with allogeneic MHC Ⅰ gene was significantly lower than which to the C57BL/6 mice bone marrow cells non modifiedConclusion:
It's maybe a way to induce immunologic tolerance in bone marrow transplantation by the means of modify the bone marrow cells with the MHC Ⅰ gene of the recipient
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
1985
Type:
Article
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