Resistance to CTL-mediated cytotoxicity of target cells transfected with Wee1 / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)2001.
Article
in Chinese
| WPRIM
| ID: wpr-675458
ABSTRACT
Objective:
To study the effect of resistance to CTL mediated cytotoxity of target cells transfected with Wee1 and treated with anti perforin antibodyMethods:
Cytotoxic T lymphocyte was obtained by mixing lymphocyte NIT culture in vitro,and proliferation index (PI) of lymphocytes was detected(MTT method) The recombinant vector pCMV Wee1Hu/GFP was transfected into mammalian cells NIT With the function of anti perforin antibody, NIT and NIT transfected with Wee1 used as target cells, CTL mediated cytotoxity was detected by LDH methodResults:
CTL proliferation was induced by mixing cell culture With anti perforin antibody, the cytolysis quantity of NIT transfected with Wee1 was leastConclusion:
Target cells transfected with Wee1 could resist CTL mediated cytotoxity, and anti perforin antibody obviously increased the effect
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2001
Type:
Article
Similar
MEDLINE
...
LILACS
LIS