The effect of receptor-associated protein in acetylcholine receptor of experimental autoimmune myasthenia gravis / 中国免疫学杂志

ABSTRACT

Objective:To investigation the effect of receptor associated protein (Rapsyn) in acetylcholine receptor (AChR) of end plate of experimental autoimmune myasthenia gravis (EAMG).Methods:Made and purified the pcDNA Rapsyn with genetic engineering technique and injected the muscle of vastus lateralis of left leg of Lewis rats, and then stimulated with electro square porator. EAMG induced by passive transfer mAb35 injected intraperitoneally, and monitor body weight and assessment clinical signs score. Sacrificed animal and removed the muscle of vastus lateralis of Lewis rats after 48 hour of induction of EAMG, measurement the AChR with radioimmunoassay (RIA) and calculated the AChR loss rate.Results:There were lower AChR loss rate found in muscle pretreated with pcDNA Rapsyn then muscle pretreated with Vector(as negative control).Conclusion:Rapsyn protein can protect the AChR of EAMG model. [