The preparation of anti-human bladder cancer/anti-VEGF bispecific antibody and in vitro study on cytotoxicity / 中国免疫学杂志

ABSTRACT

Objective:To analyse and prepare bispecific antibody(BsAb, anti human bladder cancer/anti VEGF) which could enhance the effectiveness of tumor cell killing activity.Methods:Monoclonal antibodies against human bladder cancer and VEGF were used to prepare bispecific antibody.Results:IC50 of BsAb was 10 -9.5 ,while those of antibodies against human bladder cancer and VEGF were 10 -8.9 and 10 -8.3 .Conclusion:The effectiveness of BsAb was significantly higher than that of monoclonal antibodies. [