Prediction,synthesis and characteristics of B-cell epitopes of CSFV envelope glycoprotein E2 / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)2001.
Article
in Chinese
| WPRIM
| ID: wpr-675593
ABSTRACT
Objective:
Study on characteristics of two synthesizd peptides based on CSFV E2 protein.Methods:
B cell epitopes of CSFV E2 antigen were predicted using accessibility and flexibility schemes, associated with antigenicity , secondary structure and multiple sites prediction. Two antigen peptides (Pep1 and Pep2) have been designed and synthesized and their reactivety were detected with 8 McAbs and antiserum against mE2 protein, then the peptides were conjugated with BSA and immunized rabbits respectively.Results:
Both Pep1 and Pep2 could react with antiserum and McAb A11, Pep2 could interact with McAbD5 and McAbD8. Only Pep1 BSA conjugate can stimulate high level and specific antibodies.Conclusion:
The peptide1 has good antigenicity.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prognostic study
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2001
Type:
Article
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