Impact of different organ preservation solutions on islet transplantation / 中华器官移植杂志

ABSTRACT

Objective To evaluate the impact of the different organ preservation solutions on the outcome of islet transplantation, and possibility of using hypertonic citric potassium solution as the organ preservation solution. Methods Adult human pancreata was selected and hypertonic citric potassium solution and UW solutions were used as organ preservation solutions respectively to isolate and purify human islets. Islet number and IEQ as well as the functions of the two groups were compared. Results There was no significant difference in the islet number after isolation ( P values were 0.671 and 0.716 respectively), and there was no significant difference in the islet number after purification ( P values were 0.684 and 0.767 respectively) between the two groups. After purification, there was no significant difference in the concentration of insulin secreted by islets between the two groups both under the stimulation of high concentration and low concentration glucose ( P values were 0.275 and 0.546 respectively), and no significant difference in C pep secretion ( P values were 0.126 and 0.657 respectively). Survival rate of the islets in the two groups was over 85 % . Conclusion In pancreas transplantation, hypertonic citric potassium solution could be used as pancreas preservation solution.