Study on the expression of CD28 costimulatory molecule on human lymphocytes inhibited by special siRNA / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)1985.
Article
in Chinese
| WPRIM
| ID: wpr-675759
ABSTRACT
Objective:
To investigate the expression of CD28 costimulatory molecule on human lymphocytes inhibited by siRNA.Methods:
Three different siRNA (siRNA 1,siRNA 2,siRNA 3) were designed and synthysized and transfected into freshly isolated human lymphocytes with cationic liposome.At 24,48 and 72 h post transfection,the changes of CD28 expression were detected by flow cytometry,and the changes of CD28 mRNA levels were determined by semi quantitative RT PCR.Results:
Different siRNA showed different reduction in CD28 expression.At 48 h post transfection,the degrees of reduction with siRNA 1,siRNA 2 and siRNA 3 were 22 10%?1 63%,73 50%?1 02% and 42 90%?0 89% respectively compared with the control ( P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
1985
Type:
Article
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