Protective effects of hyperpolarized cardiac preservation solution on isolated rat heart / 中华器官移植杂志

ABSTRACT

Objective To evaluate the protective effects of a novel hyperpolarized cardiac preservation solution on isolated rat heart. Methods Male Sprague Dawley rats (250~300 g, n =24) were randomly divided into three groups ( n =8 in each group) and their hearts were preserved with K H, UW, HCS solution respectively for 24 h. The isolated hearts were perfused in the Langendorff rat mode for functional evaluation before and after preservation. Lactate dehydrogenase and creatine phosphokinase enzyme leakages, contents of adenosine triphosphate and wet weights in myocardium were measured after reperfusion. Results The impairment of the left ventricle function in K H group was serious, while that in HCS and UW groups was mild. The recovery of coronary flow in HCS group was better than K H and UW groups. There were no significant differences in releases of LDH and CPK and wet weight of myocardium between UW group and HCS group. HCS group offered a less variety of pH and had the highest ATP content of myocardium among the three groups. The time, from the reperfusion to beating of heart, showed no difference between UW group and HCS group. The ultrastructural changes were slight in HCS group. Conclusions HCS solution can protect rat heart for 24 h effectively. The recovery of heart function by HCS is similar to that by UW solution. HCS solution can improve the coronary flow and acidosis and protect ATP more effectively than UW solution.