Apoptosis and expression of Fas, TNF-? and Bcl-2 in patients’serum and cerebrospinal fluid with HAM/TSP / 中华神经科杂志
Chinese Journal of Neurology
;
(12)2000.
Article
in Chinese
| WPRIM
| ID: wpr-675822
ABSTRACT
Objective To investigate the apoptosis of peripheral blood lymphocyte cells in patients with HAM/TSP and the roles of Fas/FasL system, TNF ? and Bcl 2 protein in occurrence and progression of HAM/TSP Methods The single cell gel electrophoresis (comet assay) was used to detect the apoptosis of lymphocyte cells in patients with HAM/TSP The presence of sFas, sFasL in the serum and cerebrospinal fluid (CSF) of patients with HAM/TSP was evaluated by using an enzyme linked immunosorbent assay (ELISA) The Fas, TNF ? and Bcl 2 protein expressions were quanlitatively determined by flow cytometry from 8 cases of HAM/TSP, and compared with the controls and other inflammatory neurological diseases (OIND) Results (1) The apoptotic ratio of lymphocyte cells in patients with HAM/TSP (13 63%?0 48%) was significantly increased as compared to the controls (1 63%?0 57%, P 0 05) Levels of sFas and sFasL in CSF from patients with HAM/TSP ((0 19?0 07) ng/ml and (0 24?0 08) ng/ml) were both significantly increased than those from OIND ((0 11?0 06) ng/ml and (0 01?0 01) ng/ml, P 0 05) or patients with OIND (34 84?9 63, P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Neurology
Year:
2000
Type:
Article
Similar
MEDLINE
...
LILACS
LIS