Development and application of the method for the detection of human IL-6 mRNA / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)1985.
Article
in Chinese
| WPRIM
| ID: wpr-675955
ABSTRACT
Objective:
To develop and apply a method for real-time quantifying IL-6 mRNA.Methods:
By Ficoll-Hypaque density gradient centrifugation, human peripheral blood mononuclear cells(PBMC) were isolated from whole blood treated with ethylenediaminetetraacetic acid(EDTA). Total RNA extracted from the PBMC in trizol solution was reverse transcribed to cDNA, which used as templates for fluorecent real-time quantitative PCR amplification of IL-6. PCR system consists of IL-6 primer, SBY Green Ⅰ and so on.Results:
The method is wide linear range, sensitive, specific, reproducible and simple.Conclusion:
The method can accurately quantify IL-6 mRNA.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
1985
Type:
Article
Similar
MEDLINE
...
LILACS
LIS