Detection of human cytomegalovirus infection in renal transplant recipients / 中华器官移植杂志

ABSTRACT

Objective To establish a new method for the diagnosis of active human cytomegalo- virus(HCMV)infection in renal transplant recipients and investigate its value in guiding antivial the- rapy.Methods The expression of HCMV phosphoprotein 65(HCMV pp65)antigen in peripheral blood leucocytes was detected by immunohistochemistry and catalyzed signal amplification(CSA). Results In 100 renal transplant recipients,44 were found to be positive for HCMV pp65 antigen.The mean number of antigen positive cells was(72?45)/2?10~5 WBC in 29 recipients suffering from symp- tomatic HCMV infection,while that of 15 asymptomatic patients was(46?25)/2?10~5 WBC(P