Relationship between protein level and ? mRNA,? mRNA isoforms of glucocorticoid receptor and effect of intravenous injection of methylprednisolone effect in multiple sclerosis patients / 中华神经科杂志

ABSTRACT

Objective To investigate the expression of glucocorticoid receptor(GR), GR? mRNA and GR? mRNA in peripheral blood mononuclear cells(PBMC)of patients with multiple sclerosis(MS) and healthy controls and investigate the relationship between GR protein, GR? mRNA, GR? mRNA and intravenous injection methylprednisolone(IVMP)effect.Methods GR in PBMC was measured by radioligand assays in 20 patients with relapsing-remitting MS(RRMS), 6 with the second progressive MS (SPMS)and 26 healthy controls.Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR)was used to detect the expression of GR? mRNA and GR? mRNA in PBMC of patients with MS and healthy controls.The effect of IVMP was evaluated by the expanded disability status scale(EDSS). Results(1)Before IVMP, GR in PBMC of MS was significantly lower(RRMS(3.8?0.2)?10~3 site/cell;SPMS(1.6?0.2)?10~3 site/cell)than those of controls((4.2?0.8)?10~3 site/cell, P