Expression of raf-1 in HepG2 strain stably transfected by HBx gene / 肿瘤研究与临床
Cancer Research and Clinic
;
(6)2006.
Article
in Chinese
| WPRIM
| ID: wpr-676684
ABSTRACT
Objective To investigate the effect on cell cycle of hepatitis B virus x protein and ap- proach the molecular mechanism of x protein's carcinogenesis. Methods Gene transfection mediated by the lipofectamine was used to introduce the eukaryotic expression vector of HBV x gene into human hepato- cellular carcinoma cell line HepG2(HepG2X cell).The selective medium containing G418 was used to select the cell clones which the X protein was expressed constantly.Flow eytometry was used to detect the cycle of the cells;raf-1 protein was detected by Western blot.Results X protein could be expressed on 21?10~3 loca- tion in the transfected cells,while there were no protein expressed in the control cells.The proliferation of X cells increased significantly according to MTS method(P
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Cancer Research and Clinic
Year:
2006
Type:
Article
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