Analysis of Fourteen Dyes in Hair Dying Formulations by High Performance / 环境与健康杂志
Journal of Environment and Health
;
(12)2007.
Article
in Chinese
| WPRIM
| ID: wpr-676806
ABSTRACT
Objective To develop a high performance liquid chromatography (HPLC) for the determination of fourteen dyes in hair dying formulations.Methods Dyes were separated on a amide bonded C_(16) silica column (250 mm?4.6 mm,5?m),the target analytes were ion-paired prior to HPLC analysis with elution employing methanol-0.025 mol/L phosphate buffer (pH=6.0) containing 1 g/L 1-heptanesulfonic acid sodium salt as mobile phase and detection by a photodiode array detector with the detection wavelength of 230 nm and 280 nm.The flow rate was 1.0 ml/min and the column temperature was 25℃.Results The linear range were 10-500 mg/L,the detection limits were 0.3-2.0 mg/L,the coeffieients of variation were less than 10%(except 2-amino-6-chloro-4-nitrophenol at low concentration) and the recovery rates were 71.3%-118.5%.Conclusion The methods of high performance liquid chromatography introduced in this paper is simple,rapid,accurate and is applicable to the analysis of various hair dyes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Environment and Health
Year:
2007
Type:
Article
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