DETERMINATION OF FOLIC ACID IN FORTIFIED INFANT MILK POWDER BY HPLC / 营养学报

ABSTRACT

The fortified milk powder is dissolved in 0.05M K2HPO4 buffer solution, adjust pH to 4.5, and centrifuge for 20 minutes. The supernatant is then adjusted to pH 4.0, go through CGS resin column using 10ml KC1-K2HPO4 as eluant. The eluate is collected as testing solution and injected to HPLC. Column ODS; mobile phase; 0.05M K2HPO4 CH3OH (919); ultraviolet detector 285nm. CV% is 3.3% and recovery 87%.