Analysis of the Antigens of Vibrio cholerae with Monoclonal Antibody / 第二军医大学学报

ABSTRACT

In this paper, the antigens of both Vibrio cholerae on 55 strains of El Tor and on 28 strains obtained with genetical method were studied by ELISA method using 12 strains of McAb against Vibrio cholerae (VCOMcAb). The results indicated that the 55 strains of Vibrio cholerae El Tor were expressed 11(A-K) of the recognized antigenic determinants, and fell into XⅧ(Ⅰ-XⅧ) distinct McAb reactivity types or groups, and the 28 varients of Vibrio cholerae from genetical method were expressed 9 (A-Ⅰ) of the recognized antigenic determinants, and fell into Ⅹ (Ⅰ-Ⅹ) distinct McAb reactivity types or groups. From those VCOMcAbs, the “F3” could recognize all 55 strains of Vibrio cholerae El Tor, indicating it has specificity; other 11 strains of VCOMcAb have a variety of reactivity to antigens on the 55 strains of Vibrio cholerae El Tor in both Inaba and Ogawa and their percentages of reactivity are different. This shows that these strains may have subtypes or subgroups.