In vitro study of endotoxin effects on lipid peroxidation injury of hepatocytes of rats / 第三军医大学学报

ABSTRACT

The effects of endlbtoxin on lipid peroxidation injury of rat hepa-tocytes and the protective effects of exogenous superoxide dismutase (SOD) were studied in vitro.It was found that after the isolated rat hepatocytes and endotoxin (500 ng/ml) were incubated together for 6 hours,there was an obvious increase of malondialdlehyde (MDA) level and lactic dehydrogenase (LDH) and SOD activities,and the increase of LDH and SOD activities occurred earlier than that of MDA level.Polymorphonuclear neutrophils (PMN) after activated by endotoxin could destroy the hepatocytes and increase MDA level and LDH and SOD activities which could partially be inhibited with the pretreatment of exogenous SOD.These findings suggest that endotoxin can intensify the injurious effects of PMN on hepatocytes and it can also directly induce lipid peroxdatipn injury on hepatocyes but the mechanism was not clear.