STUDIES ON THE PROTECTIVE IMMUNITY OF Sj 60-90 KD IMMUNOGEN IN SCHISTOSOMA JAPONICUM / 中国血吸虫病防治杂志

ABSTRACT

The 60-90 KD antigen of Schistosama japanicum isolated from SEA by SDS-PAGE was used to immunize female C57BL/6 mice viasubcutaneous injection route. Protective immunity against challenge infection with cercariae of S. japonicum was evaluated by the adult worm reduction rate, and the levels on humoral and cellular immunity in immunized mice.A high level of anti-SEA antibody litre in sera collected from immunized mice was detected by ELISA. The value of the adult worm reduction rate showed that there was no significant protective effect on challenge infection and no obvious difference beween immunized mice and non-immunized control groups. In addition, the data of T-cell proliferative response to ConA of spleen cells revealed that 60-90 KD antigen inhibited the response of T-cell to the mutagen. The possibility of induction of different results of immunity was discussed.