Application of dextran sulfate pretreatment in animal model of umbilical cord blood transplantation / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12)2000.
Article
in Chinese
| WPRIM
| ID: wpr-677166
ABSTRACT
Objective:
To better understand homing potentiality of hematopoietic stem cells (HSC) in human umbilial cord blood (UCB) and the mechanism of dextran sulfate (DS) mobilization.Methods:
Sub lethally irradiated or DS pretreated severe combined immunodeficient (SCID) mice were transplanted with UCB, which was cryopreserved at -80℃.Human cells in recipient mice were detected by flow cytometry and CFU GM assay from each host organ.Results:
In contrast with the controls, engraftment after irradiation or administration of DS resulted in a higher percentage of CD45 +,CD34 +,CD19 + cells produced in SCID mice. While comparison between the experimental groups, higher implantation level was obtained in the former if equivalent donor cells were used in both groups.Conclusion:
DS is a safe and effective pretreatment, which can mobilize HSC, but also vocate niches for transplanted HSC homing. [
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
2000
Type:
Article
Similar
MEDLINE
...
LILACS
LIS