Experimental studies on methionine dependence of human primary gastric cancer cells / 肠外与肠内营养
Parenteral & Enteral Nutrition
;
(6)1997.
Article
in Chinese
| WPRIM
| ID: wpr-677340
ABSTRACT
Objectives:
To study the methionine dependence (Met-dependence) of human primary gastric cancer cells in vitro when Met in the culture medicine was replaced by its precussor homocysteine(Hcy).Methods:
Fresh and sterile gaotric cancer samples and normal gastric mucosa were managed into single-cell suspension and were cultured in the Met -Hcy + and Met\++ Hcy\+- medium separately.proliferation of these cells in the two different culture media was examined by cell counter and flow cytometry (FCM).Results:
In the Met\++Hcy\+- medium,the growth of primary gastric cancer cells was inhibited,manifesting that the percentage of G 0G 1 tumor cells decreased and that of the S phase increased signiflcantly.However,normal gastric mucosal cells grew well and their cell cycles had no change.Conclusions:
Human primary gastric cancer cells are Met dependent in vitro whereas normal gastric mucosal cells have no similar results.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Parenteral & Enteral Nutrition
Year:
1997
Type:
Article
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