Preparation, identification and biological activity of rhIL-2/GM-CSF fusion protein antibodies / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12)2000.
Article
in Chinese
| WPRIM
| ID: wpr-677933
ABSTRACT
Objective:
To prepare and identify recombinant human IL 2/GM CSF(rhIL 2/GM CSF) fusion protein antibodies and to study its specificity and its effect on fusion protein biological activity.Methods:
rhIL 2 /GM CSF fusion protein was purified by DEAE Sepharose FF ion exchange chromatography. The purified protein was used to immunize rabbits for the preparation of antisera. The titer and specificity of the antisera were detected by ELISA and Dot ELISA and the biological activity by cell proliferation.Results:
The antisera not only reacted with the rhIL 2/GM CSF, IL 2 and GM CSF, but also inhibited the biological activity of the rhIL 2/GM CSF, IL 2 and GM CSF.Conclusion:
The obtained antisera can be used to study the structure and function of the rhIL 2/GM CSF.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
/
Prognostic study
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
2000
Type:
Article
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