Gene expression pattern of cervical intervertebral disc degeneration in rats / 第二军医大学学报

ABSTRACT

Objective: To investigate the gene expresssion changes in normal and degeneration cervical intervertebral disc in rats, providing information for clinical diagnosis and treatment of cervical intervertebral disease.Methods: The models of cervical intervertebral disc degeneration in rats caused by imbalance between the dynamic and static forces was established.cDNA microarray chips containing 512 cDNAs were used to investigate the gene expression pattern in cervical intervertebral disc of rat.The results were scanned and analyzed by computer image analysis,and then subjected to standardization,ratio and clustering analysis.Results: Eighteen genes expressed in cervical intervertebral disc were screened out,comprising 11 up regulated and 7 down regulated ones,respectively.This genes expression in cervical spondylosis was similar to other reports.Conclusion: Signal transduction pathways plays an important role in the process of cervical intervertebral disc degeneration.Investigation on the gene expression pattern is helpful for studying degeneration of cervical intervertebral disc pathogenesis.