The dynamic changes of the content of taurin and the activity of phospholipase A_2 in experimental models of ischemia in the canine brainstem / 第三军医大学学报

ABSTRACT

Objective To observe the dynamic changes of the content and the activity of phospholipase A 2(PLA 2) after ischemia in canine brainstem and to explore the neuroprotection of taurin and the activation of PLA 2. Methods The ischemic brainstem models were established with dog.The content of taurin and the activity of PLA 2 were determined after different durations of ischemia in the brainstem with Gold series high performance aminoacid analyzer and trace acid titration respectively. Meanwhile,the pathological changes of neurons were observed with microscope. Results Compared with those of the pseudo operation group,the content of taurin and the activity of PLA 2 in the brainstem increased significantly 30 min after ischemia in the brainstem ( P