Protective effects of (-)-epigallocatechin-3-gallate on renal ischemia-reperfusion injury / 中国药理学通报

ABSTRACT

AIM To study the protective effects of ( ) epigallocatechin 3 gallate (EGCG) against renal ischmia reperfusion injury. METHODS The renal ischemia reperfusion injury model in rat was made by means of ligating bilateral renal arteries for 60 min and then reperfusion. 10 mg?kg -1 and 40 mg?kg -1 EGCG were given by intravenation before and after operation respectively. Spectrophotometric assay were used to measure the contents of serum creatinine(Scr), blood urea nitrogen (Bun) in serum and the activity of superoxide dismutase (SOD) and Ca 2+ ATPase ,the contents of malondialdehyde (MDA), reactive oxygen species (ROS) in renal hemogenate from rats. Renal pathologic changes were also observed. RESULTS Compared with model control group , 40 mg?kg -1 EGCG group was found of significant inhibition in changes of Scr, Bun,MDA,ROS and significant increases in activity of SOD and Ca 2+ ATPase.The renal morphological injury of EGCG groups were slighter than that of model control group. CONCLUSION EGCG has protective effects aganist renal ischemia reperefusion injury via its antioxidant activity and intracellular caclcium reduction.