A TLC-HPLC method to analyze taxol in leaf of Torreya Arn.plant / 第二军医大学学报

ABSTRACT

A TLC condition has been developed to purify the extraction from the leaf of Torreya Arn. plant,then an optimized HPLC method was used to determine the amount of taxol.HPLC chromatographic conditions werecolumShim Pack CLC ODS(150 mm?6.0 mm,5 ?m);mobile phasemethanol water(66∶34);flow rate 0.5 ml/min;wavelength of the detector 227 nm;colum temperature 35℃.Using this method,we have determined the trace level of taxol in the leaf of Torreya Arn .plant successfully.There exists better linear relationship between peak height( Y ) and the concentration of detected sample( X ) within 100 500 ?g/ml.The average recovery rate is 85.7%( n =12);RSD is 7.8%.