Expression of transmembrane tumor necrosis factor receptor in rats with multiple organ dysfunction syndrome / 第二军医大学学报

ABSTRACT

Objective: To explore the expression changes of transmembrane tumor necrosis factor receptor in rats with multiple organ dysfunction syndrome(MODS). Methods: Thirty two healthy male SD rats were randomly divided into 2 groups. Sixteen rats (MODS group) were subjected to two hit to establish MODS model, the other 16 rats (control group) were without any intervention. RT PCR and immunohistochemistry methods were applied to detect the expression of mTNFR 1 at the levels of mRNA and protein. Results: mTNFR 1 was expressed in normal liver, lung and kidney whereas the most abundant expression was found in liver. Compared with normal liver tissue, the expression of mTNFR 1 in MODS group was significantly higher ( P 0.05). Serum concentration of ALT had correlation with mTNFR 1 gene expression level in liver,but no correlation existed between serum concentration of BUN and mTNFR 1 gene expression level in kidney. Conclusion: The expression of mTNFR 1 increases after the occurence of MODS, which indicates that the impact of TNF can be modulated at the level of receptor.