Construction and identification of recombinant adenovirus containing double suicide fusion gene / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12)1982.
Article
in Chinese
| WPRIM
| ID: wpr-678078
ABSTRACT
Objective:
To construct a recombinant adenovirus containing cytosine deaminase( CD ) gene and thymidine kinase( TK ) fusion gene for the gene therapy research of malignant tumors.Methods:
A recombinant cosmid containing CD and TK fusion gene was constructed, and then mixed with DNA TPC and co transfected to the 293 cells by calcium phosphate coprecipitation.Results:
The results of restriction and PCR showed the insertion was right and the recombinant adenovirus generated contained the CD and TK fusion gene without replication competent adenovirus.Conclusion:
The recombinant adenovirus generated is E1 and E3 deleted and contains double suicide gene needed, which can be further studied for gene therapy.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
1982
Type:
Article
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