EFFECTS OF EICOSAPENTAENOIC ACID IN COMBINATION WITH RETINOIC ACID ON N-RAS EXPRESSION IN HL-60 CELLS / 营养学报

ABSTRACT

Objective:To study the effects of eicosapentaenoic acid (EPA) and retinoic acid (RA) on proliferation and differentiation of HL 60 cells and the corresponding mechanisms. Methods:Flow cytometry was used to assay cell proliferation function, NBT reduction experiments to evaluate cell differentiation, and Western blot to analyse pRB expression. Results:The proliferation index (PI) in all experimental groups were all lower than that of the control , and EPA in combination with RA was the lowest one. NBT reduction experiments showed that the OD value of cells treated by EPA and RA was about 5.9 times of the control, but correspondingly by RA was 2.6 times. Moreover, p21 N ras expression of HL 60 cells were all decreased by treatment with EPA or/and RA. Conclusion:The synergistic effects of EPA and RA on proliferation and differentiation of HL 60 cells may be partly due to the change of p21 N ras expression.l proliferation function,NBT reduc