Screening of gastric carcinoma subcell line with higher metastatic potential and c-Met expression / 第三军医大学学报

ABSTRACT

Objective To screen a gastric carcinoma subcell line with higher metastatic potential and to explore the relationship between the liver metastatic potential and liver microenvironment with the screened subcell line Methods A gastric carcinoma subcell line was screened via procedures of orthotopic transplantation of subcutaneous tumor of its parent cell line SGC 7901 in nude mice and primary tissue culture of its liver metastatic focus Expressions of bFGFR, TGF?R 1 and c Met(hepatocyte growth factor receptor) in both the subcell line and its parent cell line were determined by immunohistochemistry Results A gastric carcinoma cell subcell line(named SGC 7901LM 2) was established from its parent cell line SGC 7901 with higher metastatic potential, and the expression of c Met increased significantly in the screened subcell line Conclusion The screened gastric carcinoma subcell line with higher metastatic potential is helpful for the further studies of liver metastasis of gastric carcinoma The elevated expression of c Met may be helpful to the liver metastatic potential of SGC 7901LM 2